Catalog Number - 59305-03

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by chromatographers for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from classical C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it particularly useful for laboratories operating legacy methods and compendial procedures.

59305-03 is a 300 mm x 3.9 mm ID analytical column packed with 5 µm, 125Å MicroBee C18 particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ and similar columns, making it especially valuable for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for difficult separations, while the 5 µm particle size delivers greater efficiency and sharper peak shapes than 10 µm particles. The 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L1 column is well suited for hydrophobic and neutral compounds analyzed under reversed-phase conditions. The traditional C18 chemistry provides reliable retention for a broad range of analytes and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic molecules. The combination of a longer column length and smaller particle size makes this column an excellent choice when maximum chromatographic resolution is required from a legacy C18 method.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Higher efficiency than comparable 10 µm columns

• Maximum resolution from 300 mm column length

• Improved peak shape and separation performance

• Excellent for hydrophobic and neutral compounds

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Method transfer projects

• Pharmaceutical analysis

• Impurity profiling

• Long-chain fatty acid analysis

• Lipophilic compound separations

• Complex sample separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• 5 µm particles provide higher efficiency and sharper peaks

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Extended column length provides maximum resolving power

• Excellent alternative for many USP L1 methods

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